This article is from Molecular Vision, volume 19.AbstractPurpose: The aim of this study was to quantify specific proteins deposited on daily wear silicone hydrogel lenses used in combination with multipurpose disinfecting solutions (MPDSs) by applying multiple-reaction-monitoring mass spectrometry (MRM-MS). Methods: Balafilcon A or senofilcon A contact lenses used with different MPDSs on a daily wear schedule were collected. Each worn lens was extracted and then digested with trypsin. MRM-MS... Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3580989
byTeixeira, Leandro B. C.; Buhr, Kevin A.; Bowie, Owen; Duke, Felicia D.; Nork, T. Michael; Dubielzig, Richard R.; McLellan, Gillian J.
This article is from Molecular Vision, volume 20.AbstractPurpose: To describe and validate a semi-automated targeted sampling (SATS) method for quantifying optic nerve axons in a feline glaucoma model. Methods: Optic nerve cross sections were obtained from 15 cats, nine with mild to severe glaucoma and six with normal eyes. Optic nerves were dissected, fixed in paraformaldehyde and glutaraldehyde, and processed for light microscopy by resin embedding, sectioning, and staining of axon myelin... Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3976691
byJester, James V.; Nien, Chyong Jy; Vasiliou, Vasilis; Brown, Donald J.
This article is from Molecular Vision, volume 18.AbstractPurpose: The purpose of this study was to determine the acute and long-term effects of mitomycin C (MMC) on quiescent rabbit corneal keratocytes regarding cell proliferation, myofibroblast differentiation and DNA repair. Methods: Quiescent keratocytes cultured in serum-free media were exposed to various concentrations of MMC and then treated with transforming growth factor-β (TGFβ). DNA damage was evaluated in both cultured keratocytes... Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398499
This article is from Molecular Vision, volume 19.AbstractPurpose: To investigate the effect of quinotrierixin, a previously reported inhibitor of X-box binding protein 1 (XBP1), on cell proliferation and viability in human retinal pigment epithelium (RPE) cells. Methods: Subconfluent human RPE cells (ARPE-19) were exposed to quinotrierixin for 16–24 h. Cell proliferation was determined with 3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay, hemocytometer counts, and CyQUANT... Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541046